1 reply to this topic

[irlogan]

Colleagues,
I'm having problems getting a clean product whilst trying to PCR from cDNA libraries. I'm sure the primer annealing temps. are good and the PCR seems to work well on a positive control of pure plasmid DNA. I'm also sure that my gene is expressed in this library.
Any ideas ?? Thanks in advance

irl

[Wang HQ]

Hi, Irl:

Please try a nested PCR using the sequencing primers for the cDNA library and the specific primers for the gene. This may help you.

Good luck

Wang HQ