controlling humidity in incubator?
Posted 10 December 2009 - 07:45 PM
Posted 11 December 2009 - 06:26 AM
Modern ones could have ultrasonic humidification. If not, there's an old paper, how to do it on your own:
P.W. Winston & D.H. Bates (1960): Saturated solutions for the control of humidity in biological research. Ecology 41-1: 232-237.
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
Posted 14 December 2009 - 07:06 AM
Posted 14 December 2009 - 09:02 AM
Most incubators these days are sophisticated and can be controlled within fine limits. Microgirl is right in a way that you can just stick a pan of water in there and you will humidify the incubator. HOWEVER the amount of humidification is essential and the relative amount of condensation within the incubator also essential. Droplets of water should be located in a specific part of the incubator. The more spots of condensation you get will also increase the chances of microbial contamination.
Relative humidification rates should be between 95-98%. This means that you get virtual no evaporation from TC plates/dishes.
Hope this is useful