Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

controlling humidity in incubator?


  • Please log in to reply
3 replies to this topic

#1 prof. moriarty

prof. moriarty

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 46 posts
0
Neutral

Posted 10 December 2009 - 07:45 PM

how is humidity controlled in an incubator? do most use humidity pans? are these special pans or can any container work for the water?

#2 hobglobin

hobglobin

    Growing old is mandatory, growing up is optional...

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,523 posts
95
Excellent

Posted 11 December 2009 - 06:26 AM

how is humidity controlled in an incubator? do most use humidity pans? are these special pans or can any container work for the water?


Modern ones could have ultrasonic humidification. If not, there's an old paper, how to do it on your own:

P.W. Winston & D.H. Bates (1960): Saturated solutions for the control of humidity in biological research. Ecology 41-1: 232-237.
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

#3 microgirl

microgirl

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 122 posts
0
Neutral

Posted 14 December 2009 - 07:06 AM

We've always just stuck a pan of water in the bottom. Not much "control" but it does prevent plates from drying out too quickly.

#4 rhombus

rhombus

    Rhombus/Uncle Rhombus

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 214 posts
20
Excellent

Posted 14 December 2009 - 09:02 AM

how is humidity controlled in an incubator? do most use humidity pans? are these special pans or can any container work for the water?



Most incubators these days are sophisticated and can be controlled within fine limits. Microgirl is right in a way that you can just stick a pan of water in there and you will humidify the incubator. HOWEVER the amount of humidification is essential and the relative amount of condensation within the incubator also essential. Droplets of water should be located in a specific part of the incubator. The more spots of condensation you get will also increase the chances of microbial contamination.
Relative humidification rates should be between 95-98%. This means that you get virtual no evaporation from TC plates/dishes.

Hope this is useful

Kindest regards

Rhombus




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.