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Help for some general DNA Extraction Questions


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5 replies to this topic

#1 sashay77

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Posted 10 December 2009 - 05:41 PM

Hi Guys,

Many of you seem to be having similar problems and instead of posting a reply for each one separately, I am posting a single post which might help you out....

For all of you who might be dealing with precious or difficult samples, unidentified contaminants after extraction or need to concentrate DNA, Check out an equipment called Aurora by Boreal Genomics. Its a very new one on the market and resolves a lot of issues with respect to sample size, contamination and extractions for out of the norm samples.

-Sashay

Edited by sashay77, 10 December 2009 - 05:42 PM.


#2 gogreen

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Posted 13 December 2009 - 10:43 AM

Hi Sashay77,


Many thanks for your concern!! This is the second post on the same day that you promote the same product...There are 2 points I wanted to point out...

1. I guess we have some nice people around here who are happy to share what they learnt from their umpteen mistakes and give personalized suggestions to the ones who need it so that we could stop the second person doing the same mistake again and solve it in a better way in first place! I believe there is no single equipment yet that could sense if it is processing a Human biopsy or Rat paw or a mango fruit and use the "pre-validated settings" for each...!

2. Most of them in bioforum has so many experiments to do with not much funds at their disposal, Hence we don't really think of buying an automated machine to do a few RNA/DNA isolation and spend all funds on the first step.

we will try our best to solve the problems without the Surreal..sorry, Boreal Aurora...If all of us run short of ideas, Now we know who to approach!! :)

Please do share your valuable knowledge if you can help the people around without making them try and buy this product.

Thanks

gg (I hope my fellow bio-forumers would agree with me...If not, Please correct me)

Edited by gogreen, 13 December 2009 - 10:43 AM.


#3 Maddie

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Posted 14 December 2009 - 12:02 PM

I'll be working on the Aurora soon. I'll let you know how that goes :lol:
Theory is when we know everything and nothing is working. Practice is when everything is working and nobody knows why. Here, we combine theory and practice. Nothing is working and nobody knows why.

A. Einstein

#4 sashay77

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Posted 14 December 2009 - 12:43 PM

Hi GoGreen,

1. It is not an automated machine, its more like an electrophoresis unit which performs extractions. You can only process one sample at a time on one machine.

2. The system has been successful across a variety of samples because its key strengths are contaminant rejection and concentration of low sample volumes.

3. Each extraction needs its own lysis conditions, so there is no "pre-validated" one shot cure-all for all samples. It gives you great recovery from small samples, which is one of the problems faced when using column based/PCi methods.


Hi Maddie, What are you working on??? :lol:

-Sashay

Edited by sashay77, 14 December 2009 - 01:27 PM.


#5 Adrian K

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Posted 14 December 2009 - 03:38 PM

You mean this?
http://www.borealgen...com/aurora.html

It looks like a machine to me, although might not be automated, perhaps a manual machine that you are trying to tell us?

Thanks for the sharing, I think this might be a good machine, but as gogreen said, I don't think many of us afford to have one like this. Especially someone from a poor lab like me. :lol:
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong

"It's all just DNA. Do it."---phage434

#6 Maddie

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Posted 16 December 2009 - 09:39 AM

Hi Maddie, What are you working on??? :(

-Sashay


My extraction buffer is full of inhibitors, degraded collagen etc..so I was hoping to use this electrophoresis to purify and concentrate the DNA in the center of the gel. Plus, if I'm not mistaken, in the future, you should be able to add probes in the gel, so that you'll be able to target just the sequences you're interested in. A new way of enriching basically. I'm very excited by this technology. I heard the guy who invented it at a conference and jumped on him right after :(
Theory is when we know everything and nothing is working. Practice is when everything is working and nobody knows why. Here, we combine theory and practice. Nothing is working and nobody knows why.

A. Einstein




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