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cell stainer size


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#1 usflucy

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Posted 10 December 2009 - 01:09 PM

Hi,

I'm getting splenocytes and thymocytes cells for T B cell analysis by FLOW from mice. I saw a lot of people use 100 um stainers to get single cells. We only have 40 um stainers. Can I use them?

Thank you very much.
Lucy

#2 usflucy

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Posted 11 December 2009 - 06:28 AM

Sorry, I made a mistake, it's cell strainer size

Thank you

Lucy

#3 usflucy

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Posted 14 December 2009 - 12:59 PM

We've not used any size below 70um ourselves. However, 40um apparently will work just fine (see link) for lymphocyte isolation. Hope this helps

http://www.naturepro...doptive_tra.php

I found this link by entering "strainer t and b cells isolation 40um" in http://www.CellAssays.com "Protocols and Methods" section.



Thank you very much. I appreciate your help.

Lucy




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