3 replies to this topic

[usflucy]

Hi,

I'm getting splenocytes and thymocytes cells for T B cell analysis by FLOW from mice. I saw a lot of people use 100 um stainers to get single cells. We only have 40 um stainers. Can I use them?

Thank you very much.
Lucy

[usflucy]

Sorry, I made a mistake, it's cell strainer size

Thank you

Lucy

[CellSpecific.com]

We've not used any size below 70um ourselves. However, 40um apparently will work just fine (see link) for lymphocyte isolation. Hope this helps

http://www.natureprotocols.com/2006/10/02/...doptive_tra.php

I found this link by entering "strainer t and b cells isolation 40um" in http://www.CellAssays.com "Protocols and Methods" section.

Edited by CellSpecific.com, 13 December 2009 - 09:11 AM.

[usflucy]

CellSpecific.com, on Dec 13 2009, 09:08 AM, said:

We've not used any size below 70um ourselves. However, 40um apparently will work just fine (see link) for lymphocyte isolation. Hope this helps

http://www.natureprotocols.com/2006/10/02/...doptive_tra.php

I found this link by entering "strainer t and b cells isolation 40um" in http://www.CellAssays.com "Protocols and Methods" section.

Thank you very much. I appreciate your help.

Lucy