cell stainer size
Posted 10 December 2009 - 01:09 PM
I'm getting splenocytes and thymocytes cells for T B cell analysis by FLOW from mice. I saw a lot of people use 100 um stainers to get single cells. We only have 40 um stainers. Can I use them?
Thank you very much.
Posted 11 December 2009 - 06:28 AM
Posted 14 December 2009 - 12:59 PM
We've not used any size below 70um ourselves. However, 40um apparently will work just fine (see link) for lymphocyte isolation. Hope this helps
I found this link by entering "strainer t and b cells isolation 40um" in http://www.CellAssays.com "Protocols and Methods" section.
Thank you very much. I appreciate your help.