Posted 10 December 2009 - 12:23 PM
Posted 11 December 2009 - 06:39 AM
Posted 25 December 2009 - 04:28 AM
If you're working in a fungal genetic lab, you should have a established procedure of transformation. If not, i advised you to start with something easier that transforming an siRNA vector without homology.
Normally we ecise the product for ko-vectors. In this case you have to linearise the vector backbone and pray. It works for me, but got a huge number of varying phenotypes. If i remeber correct there should be an SpeI site for linearization.
I don't know what you're target gene is, but hopefully something which you can easily detect. (Colour, Fluoresence, Morphology.....)
Recentyl i tried transformation without circular plasmid and got zero transformants.
With best regars