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Surface Sterilization method for corn


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5 replies to this topic

#1 shamasu

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Posted 10 December 2009 - 06:57 AM

Hi,
I'm trying to grow corn aseptically and I'm having problems getting rid of all of the fungus on/in the seed. I've tried various approaches with bleach (not only does it leave a lot of the seed with fungus, it also negatively affects germination rates), and hydrogen peroxide (this is even worse in killing fungus, but at least the germination rates are good).

If anybody has any tips they would be greatly appreciated. Thanks.

#2 amyweaver29

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Posted 10 January 2010 - 08:22 AM

Hello! I am also interested in this. Part of my work is to finance farmers who are growing or producing corn, particularly hybrid corn.

Thanking you in advance for the assistance.


God bless,
Amy
http://www.bewegungsmelder-info.de

#3 HomeBrew

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Posted 10 January 2010 - 12:32 PM

Ethylene oxide? UV light? (PS: I don't know anything about sterilizing seeds...).

#4 lab rat

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Posted 10 January 2010 - 09:26 PM

Have you looked at orchid culture models? Orchid growers have methods for growing clones from aseptic seeds as well as tissue.
42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia....amming_language)" target="_blank">http://en.wikipedia....amming_language)</a>, accessed 25June2009.

#5 toejam

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Posted 03 February 2010 - 11:10 AM

have you tried seed coating with captan?
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#6 shane

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Posted 07 July 2010 - 09:02 PM

i think you have to consult a botany specialist...




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