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transformation medium


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#1 sud373

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Posted 09 December 2009 - 09:08 PM

I want to prepare CCMB 80 buffer which is used in transformation. one of the constituents is Potassium acetate (pH-7.0) but we dont have KAc in lab. Can I use Na acetate instead of K acetate. will it affect the tranformation efficiency?
suddu

#2 phage434

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Posted 09 December 2009 - 09:18 PM

KOH + glacial acetic acid, adjust pH to 7.0
I wouldn't necessarily be fond of doing this with concentrated solutions, at least not without some good protection.

K2CO3 + acetic acid might be a better choice, liberating CO2.

#3 sud373

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Posted 09 December 2009 - 09:27 PM

KOH + glacial acetic acid, adjust pH to 7.0
I wouldn't necessarily be fond of doing this with concentrated solutions, at least not without some good protection.

K2CO3 + acetic acid might be a better choice, liberating CO2.

Thanx a lot buddy,
Its seems a gd idea to use K2CO3 and acetate.
Can u provide me exact details of conc of constituents for both KOH+ Ac, and K2CO3+ galcial Ac.
I will try in both way.

Thanx :lol:
suddu




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