I am trying to express the bovine presenilin-1-associated protein (PSAP). I have cloned it under the CMV promoter and in frame with the HA tag. I have confirmed the desired clone by restriction enzyme analysis and DNA sequencing of the full length gene including the junctions. I had transfected it along with positive control plasmids in to 293T cells. Western blot is giving me nice band with anti-HA antibody in the positive control well. Do you guys have any experience with PSAP. I had tried the different time poins after transfection but it is making no difference.
Your valuable suggestions/help is highly needed.
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Bovine presenilin-1-associated protein (PSAP)
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