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electroblotting of DNA ...doesit work with large fragments?

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#1 anonymous



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Posted 19 January 2001 - 10:00 PM

Hi All,Is it possible to blot large DNA fragments using electro blotting? I used a 6% native PAGE with depurination. My fragment size is ~10kb. Do you think size does matter?!I used the biorad apparatus.It didnt work of course.If the DNA can move down, why cant it move across? Any thoughts?

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