4 replies to this topic

[noelmathur]

Hello folks,

My BIG stock of home-made gel loading dye is over and I would like to make a fresh stock. In all ideal world, I would like to make something thats as good as Qiagen gel loading dye. I was wondering if anybody has a good recipe of this gel loading dye? I will be glad if you share your favorite loading dye recipe (5X, 6X and 10X preferred).

Thanks in advance.

[phage434]

15% Ficol type 400 dissolved in TE
Just enough Orange G dye to turn it medium dark orange color
Store at room temperature.

[noelmathur]

phage434, on Dec 9 2009, 03:53 PM, said:

15% Ficol type 400 dissolved in TE
Just enough Orange G dye to turn it medium dark orange color
Store at room temperature.

Thanks,
Whats the concentration of this stock? Is it 5X?

[Vini]

noelmathur, on Dec 11 2009, 08:02 PM, said:

phage434, on Dec 9 2009, 03:53 PM, said:

15% Ficol type 400 dissolved in TE
Just enough Orange G dye to turn it medium dark orange color
Store at room temperature.

Thanks,
Whats the concentration of this stock? Is it 5X?

i prepare by adding 4 gm sucrose n 2.5 mg bromophenol blue in 10 ml TE....that wud b 6X...

[phage434]

I use mine at 6x, but this is obviously non-critical.