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Gel loading dye recipe


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4 replies to this topic

#1 noelmathur

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Posted 09 December 2009 - 06:28 AM

Hello folks,

My BIG stock of home-made gel loading dye is over and I would like to make a fresh stock. In all ideal world, I would like to make something thats as good as Qiagen gel loading dye. I was wondering if anybody has a good recipe of this gel loading dye? I will be glad if you share your favorite loading dye recipe (5X, 6X and 10X preferred).

Thanks in advance.

#2 phage434

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Excellent

Posted 09 December 2009 - 06:53 AM

15% Ficol type 400 dissolved in TE
Just enough Orange G dye to turn it medium dark orange color
Store at room temperature.

#3 noelmathur

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Posted 11 December 2009 - 05:32 AM

15% Ficol type 400 dissolved in TE
Just enough Orange G dye to turn it medium dark orange color
Store at room temperature.


Thanks,
Whats the concentration of this stock? Is it 5X?

#4 Vini

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Posted 11 December 2009 - 06:35 AM

15% Ficol type 400 dissolved in TE
Just enough Orange G dye to turn it medium dark orange color
Store at room temperature.


Thanks,
Whats the concentration of this stock? Is it 5X?



i prepare by adding 4 gm sucrose n 2.5 mg bromophenol blue in 10 ml TE....that wud b 6X...

#5 phage434

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Excellent

Posted 11 December 2009 - 04:07 PM

I use mine at 6x, but this is obviously non-critical.




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