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Yeast One Hybrid

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#1 anonymous



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Posted 17 February 2001 - 10:00 PM


I have encounter a lots of problems with the construction of yeast strain using yeast one hybrid kit from Clontech. The plasmid I have constructed refused to be digested. The plasmids involved are placZi, pHisi, pHisi-1.Have anyone face the same problem. I would like to know which lab is working with one-hybrid.

Thank You.

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