Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

how to check the quality of DNA?


  • Please log in to reply
4 replies to this topic

#1 susanna

susanna

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 79 posts
0
Neutral

Posted 08 December 2009 - 09:13 AM

Hi,

i have a problem with the quality of my DNA. This would be important for doing qPCR (background).
how can i check the quality? Any suggestion?

greetz,

susan

#2 lab rat

lab rat

    Why does a science forum not have pictures of mice and rats?

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 245 posts
7
Neutral

Posted 08 December 2009 - 09:39 AM

Hi susanna,

Just run an agarose gel (1%) with a 25 ng or so of gDNA. Electrophorese and stain with EtBr. A smear will indicate degraded gDNA.

regards,

lab rat
42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia....amming_language)" target="_blank">http://en.wikipedia....amming_language)</a>, accessed 25June2009.

#3 arc1

arc1

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 12 December 2009 - 03:40 AM

Hi
You could also record a UV spectrum....to get an idea of protein contamination..OD260/280 between 1.7 and 2 would mean that DNA quality is good.
All the best

#4 susanna

susanna

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 79 posts
0
Neutral

Posted 23 December 2009 - 01:50 AM

Hi
You could also record a UV spectrum....to get an idea of protein contamination..OD260/280 between 1.7 and 2 would mean that DNA quality is good.
All the best



thanks

#5 susanna

susanna

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 79 posts
0
Neutral

Posted 23 December 2009 - 01:53 AM

so which device do you suggest for UV spectrum?




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.