Hi,
i have a problem with the quality of my DNA. This would be important for doing qPCR (background).
how can i check the quality? Any suggestion?
greetz,
susan
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4 replies to this topic
#2
lab rat
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Why does a science forum not have pictures of mice and rats?
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Posted 08 December 2009 - 09:39 AM
Hi susanna,
Just run an agarose gel (1%) with a 25 ng or so of gDNA. Electrophorese and stain with EtBr. A smear will indicate degraded gDNA.
regards,
lab rat
Just run an agarose gel (1%) with a 25 ng or so of gDNA. Electrophorese and stain with EtBr. A smear will indicate degraded gDNA.
regards,
lab rat
42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia....amming_language)" target="_blank">http://en.wikipedia....amming_language)</a>, accessed 25June2009.
#3
arc1
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Posted 12 December 2009 - 03:40 AM
Hi
You could also record a UV spectrum....to get an idea of protein contamination..OD260/280 between 1.7 and 2 would mean that DNA quality is good.
All the best
You could also record a UV spectrum....to get an idea of protein contamination..OD260/280 between 1.7 and 2 would mean that DNA quality is good.
All the best
#4
susanna
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Posted 23 December 2009 - 01:50 AM
arc1, on Dec 12 2009, 03:40 AM, said:
Hi
You could also record a UV spectrum....to get an idea of protein contamination..OD260/280 between 1.7 and 2 would mean that DNA quality is good.
All the best
You could also record a UV spectrum....to get an idea of protein contamination..OD260/280 between 1.7 and 2 would mean that DNA quality is good.
All the best
thanks
#5
susanna
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Posted 23 December 2009 - 01:53 AM
so which device do you suggest for UV spectrum?
