Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

flexiPERM slide


  • Please log in to reply
No replies to this topic

#1 toprak

toprak

    member

  • Active Members
  • Pip
  • 9 posts
0
Neutral

Posted 08 December 2009 - 07:50 AM

hello everyone,

I am thinking of purchasing flexiPERM slide to grow cells on compartments on microscope sides, so I can do 8 different immunofluerescence staining afterwards. I have some questions though for people who use this.

1. Does it really make hydrophobic compartments as claimed in the catalogs? Or do I need to frame the compartments with hydrophobic pen before fixation?

2. Do you use it with Superfrost slides? I never grew cells on slides, only grew them on gelatin coated coverslips, so do I need to coat the slides before seeding the cells? and if I need to coat them when should I do the coating, before or after putting the flexiPERM?

and I would be very glad if flexiperm users can share their opinion.

thanks a lot!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.