Transfection: How long until translation starts?
Posted 08 December 2009 - 07:19 AM
does anybody of you know how long the duration from cell transfection (HeLa, 6kb plasmid) until translation start is? its because we got very different results in luciferase activity between o/n incubation and 4h incubation, respectively, before adding antibiotics. we use Roche FuGene for transfection.
Posted 08 December 2009 - 03:34 PM
Posted 09 December 2009 - 12:48 AM