2 replies to this topic

[anonymous]

I was wondering if anyone has tried solubilising an insoluble protein with a NDSB.  I am going to try it, but many people around my lab are sceptical about its success.  I would like to hear any stories from anyone who has tried them.

Thanks, Marty.

[anonymous]

I have had great success with NDSB.  I overexpress protein in baculovirus and had great difficulty with solubility.  NDSB 256 increased my yield approx 60-80% of resolubilization with sarkosyl alone.

[anonymous]

I have recently found (the hard way!) that NDSB from commerical sources must be further purified through a mixed bed ion exchange resin like TMD-8, before use in refolding

Otherwise, it still works great for my nuclear DNA binding protein.