Growing bacterial culture
Posted 07 December 2009 - 08:40 PM
Posted 09 December 2009 - 11:47 AM
Maybe the stock is dead? Try to culture it in flasks instead of falcon tubes?
Posted 09 December 2009 - 10:25 PM
Did you loosen your falcon tube cap?
Also, you revive and directly subculture on plate then only you subculture in broth? I guess it is better to subculture in broth for them to grow, then only select/ culture it on plate. It would be much more easy for bact to grow in broth.