2 replies to this topic

[somi]

I am doing 2D gel electrophoresis for E.coli. some one know what is the best method for preparing protein sample from E.coli .and is there any one know about how i can desalting my sample without use ready kit?

Edited by somi, 07 December 2009 - 06:47 PM.

[braincow]

You can find standard protocols just about anywhere. The Amersham 2D Electrophoresis handbook (pdf) has pretty good information on running 2D gels with bacterial proteins.

For desalting, I usually use the 2D cleanup kit from GE, which is widely used. If you prefer, you could google "TCA/acetone precipitation" and find a protocol, as the cleanup kit is basically this.

[somi]

braincow, on Dec 10 2009, 11:53 PM, said:

You can find standard protocols just about anywhere. The Amersham 2D Electrophoresis handbook (pdf) has pretty good information on running 2D gels with bacterial proteins.

For desalting, I usually use the 2D cleanup kit from GE, which is widely used. If you prefer, you could google "TCA/acetone precipitation" and find a protocol, as the cleanup kit is basically this.

Thanks ,the handbook was very useful and i will try to TCA.