Posted 16 December 2009 - 10:25 AM
By looking at PubMed, I found this:
Biochem Biophys Res Commun. 1997 Oct 9;239(1):257-60.
Isolation and characterization of mitochondrial DNA-less lines from various mammalian cell lines by application of an anticancer drug, ditercalinium.
Inoue K, Takai D, Hosaka H, Ito S, Shitara H, Isobe K, LePecq JB, Segal-Bendirdjian E, Hayashi J.
Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
Since ethidium bromide was not effective in mouse cell lines for isolating mitochondrial DNA (mtDNA)-less cells (rho zero cells), we examined whether an anticancer drug, ditercalinium (DC), which has been shown to exclude mtDNA from mouse cell lines, could be effective in various mouse and human cell lines. We found that after DC treatment rho zero cells could be isolated from all cell lines of mouse or human origin tested. Moreover, these rho zero cells maintained ability to receive exogenously imported mtDNA and allow its replication and gene expression. These observations suggest that DC eliminates mtDNA from mouse and human cells without affecting the property to receive exogenous mtDNA. Therefore, DC could be applicable to cell lines expressing various differentiated phenotypes for studying whether mtDNA plays a significant role in expression of phenotypes by manipulating mtDNA elimination and reintroduction.
I guess you could use PCR and use a rho zero cell as a control?
Theory is when we know everything and nothing is working. Practice is when everything is working and nobody knows why. Here, we combine theory and practice. Nothing is working and nobody knows why.