2 replies to this topic

[tom43]

Hi,

I´ll try to test some new antitumoral compounds in cytotoxicity assays. Hopefully these compounds will have an antiproliferative activity against p53 Null cells. Is there any matched-pair cell line available so we can test our compounds on p53 positive tumor cells and p53 mutated/Null cells? Thanks in advance!  

Tom

[bob1]

There's some HCT116 cells around that are isogenic (I think), though they do contain some of the isoforms of p53, so p53 may still have partial function.

[laurequillo]

You have MEF p53 (-/-) and MEF wt. You can use as well H1299 p53 null cells