4 replies to this topic

[ganesh]

I have tissue culture plates with flat bottom for suspension culture from Jet Biofil. Can i use these plates for adherent cancer cell lines or is there any treatment that i should do to make plates  adherent so cells will adhere.

[mtrnbh]

many adherent cell lines can be cultured in ordinary plates without any need to special treatment of the plate.

[bob1]

coat the wells in gelatin or poly-L-lysine.

[ganesh]

mtrnbh, on Dec 6 2009, 11:30 PM, said:

many adherent cell lines can be cultured in ordinary plates without any need to special treatment of the plate.

Thanks for your reply and i am going to use HEPG2 cell line can it be ued. And the plates are made up fron poly styrene and can it be used for adherent cell lines.

[bob1]

Actually cell culture plates are specially charged polystrene - so they should work fine, though the charge may not be right for culture.