Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

About tissue culture 96 well plates


  • Please log in to reply
4 replies to this topic

#1 ganesh

ganesh

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 05 December 2009 - 10:20 PM

I have tissue culture plates with flat bottom for suspension culture from Jet Biofil. Can i use these plates for adherent cancer cell lines or is there any treatment that i should do to make plates adherent so cells will adhere.

#2 mtrnbh

mtrnbh

    member

  • Active Members
  • Pip
  • 23 posts
0
Neutral

Posted 06 December 2009 - 09:00 AM

many adherent cell lines can be cultured in ordinary plates without any need to special treatment of the plate.

#3 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,831 posts
415
Excellent

Posted 06 December 2009 - 03:01 PM

coat the wells in gelatin or poly-L-lysine.

#4 ganesh

ganesh

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 06 December 2009 - 07:21 PM

many adherent cell lines can be cultured in ordinary plates without any need to special treatment of the plate.

Thanks for your reply and i am going to use HEPG2 cell line can it be ued. And the plates are made up fron poly styrene and can it be used for adherent cell lines.

#5 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,831 posts
415
Excellent

Posted 07 December 2009 - 02:53 PM

Actually cell culture plates are specially charged polystrene - so they should work fine, though the charge may not be right for culture.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.