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Do you add 10% Glycerin in your IP Lysis Buffer


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#1 AllenChiu

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Posted 04 December 2009 - 07:01 PM

It seems that Glycerin can protect the interaction of protein while rotating or something like that by making the solution more sticky??
But will it also reduce the efficiency of antibody-protein interaction as it makes the solution more sticky?
So what is your choice?
By the way,when I say Lysis Buffer,I mean NP-40 Lysis Buffer.
And do you adjust the pH to 7.2 or 7.4 or 8.0?hehe

#2 mtrnbh

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Posted 06 December 2009 - 09:05 AM

I haven't used glycerin, I use 0.5% triton X-100 and I always get very good results in IP. by the way I adjust the pH to 7.4

#3 AllenChiu

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Posted 06 December 2009 - 07:30 PM

I haven't used glycerin, I use 0.5% triton X-100 and I always get very good results in IP. by the way I adjust the pH to 7.4

Well,Thanks




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