Do you add 10% Glycerin in your IP Lysis Buffer
Posted 04 December 2009 - 07:01 PM
But will it also reduce the efficiency of antibody-protein interaction as it makes the solution more sticky?
So what is your choice?
By the way,when I say Lysis Buffer,I mean NP-40 Lysis Buffer.
And do you adjust the pH to 7.2 or 7.4 or 8.0?hehe
Posted 06 December 2009 - 09:05 AM
Posted 06 December 2009 - 07:30 PM
I haven't used glycerin, I use 0.5% triton X-100 and I always get very good results in IP. by the way I adjust the pH to 7.4