Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Vector map for pMH1


  • Please log in to reply
2 replies to this topic

#1 rocketfan86

rocketfan86

    member

  • Active Members
  • Pip
  • 26 posts
0
Neutral

Posted 04 December 2009 - 02:55 PM

Hi everyone. I am in a new lab and i have been given a plasmid which contains a gene of unknown function. All i know is that it is inserted in a vector called pMH1. However, there is no detailed vector map and all that it says about this plasmid is that the gene was inserted by PIPE cloning.

I have grown a culture and mini-prepped for the plasmid and i would like to figure out a way to digest out the gene to make sure the integrity of the plasmid is ok.

Does anyone have a map or link of pMH1? Any help is appreciated!

#2 pDNA

pDNA

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 493 posts
14
Good

Posted 05 December 2009 - 02:48 PM

If it is a plasmid for plant genetics try to contact www.meta-helix.com ...they should know more about that plasmid!

Regards,
p

#3 christy

christy

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 40 posts
2
Neutral

Posted 08 December 2009 - 02:12 AM

http://dnasu.asu.edu...do?vectorid=371

the site for pMH1 vector details.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.