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Checking Plasmid Expression


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#1 sihyunie

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Posted 04 December 2009 - 07:33 AM

I have a plasmid with dual T7 promoters to express both strands of a 300 bp insert.
I transformed BL21 with this construct and I want to make sure that both strands are being expressed.
Is there any way to know if expressed RNAs are staying as 2 separate single strands or forming a double strand?

#2 jangajarn

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Posted 29 December 2009 - 06:14 PM

run it on a gel before and after heat denaturation?




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