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LINE-1 detection by HRM method


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#1 thitima

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Posted 03 December 2009 - 09:00 PM

Hi,

Who are study about LINE-1 methylation ? I would like to ask you about
the detection method for LINE-1 HRM (high resolution melting). It possible, or not?
Because LINE-1 sequences are spread in whole genome sequence,
can I detect that sequence for tumor biomarker.

Thank you for your help in advance.

Regard,
Thitima

#2 lalehhabibi

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Posted 04 December 2009 - 02:19 AM

Hi

Did you see Coufal NG et al. article appeared in Nature. 2009 Aug 27;460(7259):1127-31. may be you can find your answer.

a question: do you know how much a plasmid on filter paper is stable?




View Postthitima, on Dec 4 2009, 09:30 AM, said:

Hi,

Who are study about LINE-1 methylation ? I would like to ask you about
the detection method for LINE-1 HRM (high resolution melting). It possible, or not?
Because LINE-1 sequences are spread in whole genome sequence,
can I detect that sequence for tumor biomarker.

Thank you for your help in advance.

Regard,
Thitima






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