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Beta-actin with multiple banding


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#1 Sticks

Sticks

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Posted 03 December 2009 - 10:29 AM

I have three blots. One with beta-actin, one with phosph AKT and AKT. Right now I'm having trouble with the beta-actin. On the blot it looks as if every lane has three seperate bands. I've used this specific beta-actin on other blots and it works fine. It is a polyclonal antibody. I'm not sure what to do next. Should I re-hybridize and see if it was just a fluke or should I just rerun the gel? Oh and the PhophoAKT and AKT show little to no binding (but, didn't want to open up that can of worms just yet)




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