Why spin down and resuspend the bacteria before adding lysis buffer is neccessary?
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Posted 03 December 2009 - 04:18 AM
yoyoyoyo, on Dec 3 2009, 04:01 AM, said:
Why spin down and resuspend the bacteria before adding lysis buffer is neccessary?
Probably because the lysisbuffer would be diluted by the broth and because the broth contains a lot of protein which can be removed that way.
