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yeast transformation question...


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#1 anonymous

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Posted 21 November 2001 - 10:00 PM

use about 1ug of the crude DNA, that will do. If you use too much then efficiency starts dropping.

cheers

Cagan


#2 anonymous

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Posted 21 November 2001 - 10:00 PM

Hi

I'm transforming yeast using a standard LiAc/PEG method (if there is such a thing!). Does anyone have information on the purity of DNA needed for such a transformation...is a crude ispropanol pptn from lysed e.coli OK, or is an actual mini/midiprep needed. Also, is there a maximum amount of DNA you can use in a transfomation or is it a case of more the merrier?

Cheers

Chris





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