Posted 01 December 2009 - 11:30 PM
My stable cell lines grows slower than the parental cell line. I want to know where this reduced growth rate is due to reduction in celll prliferation or is it due to induction of apoptosis. Does this question seem logical or is it understaood that cells that grow sloer have high apoptosis? Sorry just wnat to know if i'm asking a silly question.
Posted 22 December 2009 - 01:27 PM
Posted 22 December 2009 - 03:16 PM
MTT can be used for proliferation studies, but not for assessment of apoptosis, necrosis or autophagy. For each of these you need to assay for particular pathways, such as caspase 3 for apoptosis. Autophagy can be assessed by FACS using acridine orange.
Edited by bob1, 22 December 2009 - 03:18 PM.