Extracting precious tissue from too large of volumes of RNAlater
Posted 01 December 2009 - 01:24 PM
Posted 02 December 2009 - 08:51 AM
genius does what it must
i do what i get paid to do
Posted 02 December 2009 - 11:00 AM
then heaven will be yours, before you meet your end
Posted 02 December 2009 - 11:55 AM
Posted 02 December 2009 - 02:16 PM
Cells normally dont pellet effectively in RNAlater. The best way is to just add equal volume of PBS to the solution and spin it...I normally do a 9k rpm for 3 minutes...
Its better to take a maximum of 1 ml per tube during centrifugation, else you end up with some cells over the wall which would be disturbed when pipetting the solution...Just to make sure, keep the supernatent and spin once more
Hope this helps
Posted 08 December 2009 - 12:28 PM
With only about 8000 cells, you may be sedimenting the cells, but there isn't enough there to form a visible pellet. What do you want to use the cells for now?
the ultimate goal is to use for microarray!