Expired GE Hybridization Kit
Posted 01 December 2009 - 09:57 AM
has anyone tried using an expired (approx. 9 months ago) Gene Expression Hyb kit (Agilent)? I'm aware that it is still cheaper to buy a fresh one than to waste a microarray slide, and it is officially not recommended, but if it could be OK (components stored properly, like the redissolved Blocking Agent at -20C), it would be nice to use it...
Thanks for any advice.
Posted 01 December 2009 - 06:47 PM
hmm...tough call for you. I bet the kit's still fine, but nothing's a bigger waste of time and money than an experiment that has to be repeated.
Posted 02 December 2009 - 04:19 AM
if the components are stored well it should be good. I have tried with some old stuff and it used to work without any problem. If u have more of the lyophilized blocking agent, redissolve them...or use the one stored at -20.
The fragmentation buffer and hyb buffer are quite stable and can be used at a later time without any problem
Posted 02 December 2009 - 04:41 AM
thanks a lot! This helped me to decide and I'll give it a go! Fingers crossed!
Posted 02 December 2009 - 04:43 AM
You're doing it at your own risk. I cannot guarantee the success
all the best
Edited by gogreen, 02 December 2009 - 04:43 AM.
Posted 02 December 2009 - 08:44 AM
i am currently using a kit that expired the end of march and another that expired in july (all agilent ge kits).
genius does what it must
i do what i get paid to do
Posted 03 December 2009 - 07:29 PM
Best luck!!!! i m sure it will be fine!!
do tell us wat happened!!??!!
Posted 04 December 2009 - 03:50 AM
all the best!
Posted 04 December 2009 - 07:47 AM
Posted 04 December 2009 - 08:37 AM
Posted 06 December 2009 - 04:38 AM
The results which I got using arrays which were stored for over a year and a half from the date of receipt of arrays, were not quite promising...I think it affects the slide chemistry and after hybridization the probes looked stripped! I was just trying a hyb on a very old array just out of curiosity and it turned out to be bad(The arrays were stored dessicated at dark as per recommendations). So if you are using arrays which came long time ago and get a bad result, its likely to be because of the bad array than the bad reagents!
Edited by gogreen, 06 December 2009 - 05:55 AM.
Posted 10 December 2009 - 05:38 AM
Posted 11 December 2009 - 10:21 PM
That is good news!! I was talking about Agilent arrays too..For quite old arrays, you sometimes see some kind of degeneration of the probes..The probes are no more completely dense, they tend to show non uniformity in the spot pattern rather than showing a perfect circle. If the image looks good, it should be fine..