Jump to content


- - - - -

stored cytokines from supernatant cell culture


3 replies to this topic

#1 ziko

    member

  • Active Members
  • Pip
  • 7 posts

Posted 01 December 2009 - 09:17 AM

;) Dear all

i need advice from every one who has exprince about cytokines stored after collection from supernatant of cell culture(monocytes) untill mesaurment by ELISA.
can i store the supernatant which contain cytokines at -80 oC or -20 oC? B)


ziko

#2 wind

    member

  • Active Members
  • Pip
  • 9 posts

Posted 01 December 2009 - 05:37 PM

-20 will be ok!

#3 miBunny

    Veteran

  • Active Members
  • PipPipPipPipPip
  • 130 posts

Posted 03 December 2009 - 05:58 PM

ditto -20!

#4 labprincess

    member

  • Active Members
  • Pip
  • 14 posts

Posted 14 January 2010 - 10:43 PM

-20 should be fine. However, be very careful to minimize freeze thaws or your cytokines will crap out!





Home - About - Terms of Service - Privacy - Contact Us

©1999-2011 Protocol Online, All rights reserved.