Is there any way i get rid of coomassie blue stain from my electroeluted protein?
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Posted 21 July 2004 - 01:01 AM
hai,
instead of coomassie brilliant blue for staining during electroelution
u can use CuCl2 (cupric chloride) for negative staining
which stains the whole gel except the protein and destain by
tris and edta
all the best
instead of coomassie brilliant blue for staining during electroelution
u can use CuCl2 (cupric chloride) for negative staining
which stains the whole gel except the protein and destain by
tris and edta
all the best
M. Naga Leelaram,
PhD student,
Indian Institute of Science,
Bangalore
India
PhD student,
Indian Institute of Science,
Bangalore
India
