I need to radioactive label proteins (35S) and then perform an IP of my protein of interest. I wanna express the amount of IPed protein as a percentage of the total labelled protein. The problem is that I cannot simply count the cells in the scintillation counter since the Met-tRNA represents such a large pool in the cells which would lead to large errors in my analysis.
My question: is their an elegant way to remove the tRNA from the cell lysate? Or do I need to TCA precipitate my proteins and filter them through a filter membrane? Is their a kit I can use for that?
Any help is more than welcome (if it is reasonable, money for a kit would not be an issue).
Thanks in advance!
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