1) I examined an isolated colony of a bacterial species from my nose - and it turns out it is resistance to certain antibiotics while susceptible to others. Would I expect a second isolate from the same source (my nose) to share similarities in antibiotic sensitivity with my current isolate?
Attempt: i am thinking that the answer is no, since there are many different bacteria that are part of our normal flora on the skin - thus the second isolate may be of a different species which may have different resistance/susceptibility to different antibiotics. so i don't think location on the human body plays a factor in determining which organisms become resistant/susceptible - but i am not sure about this.
2) I ran a agarose gel electrophoresis of a plasmid of a Staph. captis species along with a control Staph. schleiferii species. I am asked: is it likely that any of the antibiotic resistances of Staph. captis and the control Staph. schleiferii would be encoded by genes located on a plasmid? How might you determine if this were truly the situation?
first step I think is involved is to see if Staph schleiferii and Staph. captis have any similar antibiotic resistances or susceptibilities. if they do, we check the agarose gel electrophoresis photo of the plasmids from the two different species, and check whether they have any same sized plasmids - if so, it might be possible that they possess the same plasmid - and if they also have resistance for the same antibiotics then this will further support that possibility .
i also thought we can do rapd pcr on one specific resistance gene and check if it is on the plasmid...it will show up on gel if not then nothing on gel saying i will be on the host chromosome genome
Edited by ydogyy1, 29 November 2009 - 07:58 PM.