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AdEasy Vector System: Why PacI digestion?


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#1 gibbs

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Posted 29 November 2009 - 12:10 PM

Hello,

why do I have to perform a PacI digestion of the purified vector prior to transfection of 293 cells?
What happens when skipping this step and directly transfect the cells?

thanks <_<
gibbs

#2 bob1

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Posted 29 November 2009 - 04:06 PM

I don't actually know, but I am guessing that the digest linearizes the plasmid, meaning your transfection is more likely to work - check the product manual, it will have a description of the process.

Edited by bob1, 29 November 2009 - 04:07 PM.





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