I want to know if a preincubation with the anti-oxidant glutathione (GSH) will abolish drug-induced ROS production in my cells. I'm searching in the literature for the right conditions to test this. As far as I found, a 30' preincubation (before I add the drug) is enough. I found different concentrations, but I think I'll use 5 mM. However, if someone has experience with this, please post here.
Also, what is the best solvent for my GSH stock solution? dH2O? Do I have to set the pH for this solution? Can I store this stock at -20º C?
Edited by cardosopedro, 29 November 2009 - 10:20 AM.