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his-tagged protein purification

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#1 anonymous



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Posted 22 January 2001 - 10:00 PM

We have some difficulties to elute a 27 kD His-tagged protein from a nickel purification column We checked the protein is greatly expressed in our extract by western blot but it seems to stich to the beds! Where is the bug? Does a super elute buffer exist?could the extract concentration be too high?Thanks

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