Posted 27 November 2009 - 08:28 AM
I'm just beginning the real time pcr and have some questions about the primer design. i was told that primers must be designed to span the intron to eliminate the gDNA contamination. but i'm a little confused about whether designing one of the primers spans the intron(exon-exon junction) or the product of the primers spans the intron. which one is better?
So please help me solve the question
Posted 27 November 2009 - 08:59 AM
Posted 27 November 2009 - 10:45 AM
Edited by tea-test, 27 November 2009 - 10:46 AM.