PFA 4%, PBS-Tween-BSA .....
Posted 26 November 2009 - 03:07 PM
Do you made fresh PFA 4 %, PBS-Triton and PBS-tween?? Or could be stored?
I read PFA could be stored at -20°C, and defroze just ones. But what about PBS-tween and PBS-BSA-tween?
For a cell culture immunofluorescence, protocol says: fix with PFA 4 %, but a labmate told me to use PFA 4%, then EtOH, then acetone.
Which is better and why?
Thank you very much for your advice!!!
Posted 28 November 2009 - 08:21 AM
Posted 29 November 2009 - 04:17 PM
Posted 08 December 2009 - 07:55 AM