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Wash buffer pH

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2 replies to this topic

#1 prof. moriarty

prof. moriarty


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Posted 26 November 2009 - 12:41 AM

I am doing an ELISA with this kit - http://www.ebioscien..._88/88-7324.pdf
It doesn't come with the wash buffer. It recommends a pH of 7.4, tween 20 0.5%, pbs.
I have found a wash buffer that meets all of those requirements except that it is pH 7.5.
Will this make a big difference or is it okay?


#2 Gerard



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Posted 26 November 2009 - 02:43 AM

It will be okay, ^_^
and can you garantee that the pH measurement is 100% OK
Ockham's razor
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".

#3 Prep!


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Posted 26 November 2009 - 02:52 AM

As far as i understand the pH is chosen as Generally after binding of antigen and antibody or both we wash with buffer which could be in the range of ph: 7.0-7.5.
this is considered good because it's almost neutral pH, where there is least chance of disturbing the binding of antigen, antibody or both.
May not be compulsary but there has to be some logic or else all wont use that pH universally!!!
Best luck!
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