Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Wash buffer pH


  • Please log in to reply
2 replies to this topic

#1 prof. moriarty

prof. moriarty

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 46 posts
0
Neutral

Posted 26 November 2009 - 12:41 AM

Hello,
I am doing an ELISA with this kit - http://www.ebioscien..._88/88-7324.pdf
It doesn't come with the wash buffer. It recommends a pH of 7.4, tween 20 0.5%, pbs.
I have found a wash buffer that meets all of those requirements except that it is pH 7.5.
Will this make a big difference or is it okay?


Thanks!


#2 Gerard

Gerard

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 124 posts
0
Neutral

Posted 26 November 2009 - 02:43 AM

It will be okay, ^_^
and can you garantee that the pH measurement is 100% OK
Ockham's razor
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".

#3 Prep!

Prep!

    Am I me???!!!

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 517 posts
4
Neutral

Posted 26 November 2009 - 02:52 AM

As far as i understand the pH is chosen as Generally after binding of antigen and antibody or both we wash with buffer which could be in the range of ph: 7.0-7.5.
this is considered good because it's almost neutral pH, where there is least chance of disturbing the binding of antigen, antibody or both.
May not be compulsary but there has to be some logic or else all wont use that pH universally!!!
Best luck!
Support bacteria - They are the only culture some people have!!!
Cheers!!!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.