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PLASMA RNA extraction


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#1 SF_HK

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Posted 25 November 2009 - 11:43 PM

HI,

I will soon be extracting RNA form plasma using SV total RNA isolation kit (Promega). I understand its quite difficult to get a good yield. I will be using 20oul of plasma volume for the RNA extraction. This is my first time. All advice/suggestions would be welcomed.

#2 molgen

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Posted 26 November 2009 - 02:55 AM

I use the SV total RNA isolation kit freequently.
It's a very straight forward protocol.
Not much place to go wrong with.
Just follow the instructions.

BTW, if you're apprehensive about the yield, you might want to use a micro prep extraction kit.
When I have to extract from little fragments (10-60mg) I use the zymo ZR RNA MicroPrep




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