3 replies to this topic

[shashababy]

Hey everyone:
      I have some troubles in my yeast two hybrid experiments.  Becasue my bait is self-activation , even after contransformed with prey, should all  the results   turn blue  in Theoretically? But when i cotransformed  my bait and preys,  the result is not all turn blue. （the same bait vs several preys）    Why?

[eldon]

shashababy, on Nov 25 2009, 04:42 AM, said:

Hey everyone:
      I have some troubles in my yeast two hybrid experiments.  Becasue my bait is self-activation , even after contransformed with prey, should all  the results   turn blue  in Theoretically? But when i cotransformed  my bait and preys,  the result is not all turn blue. （the same bait vs several preys）    Why?

the prey interacts to inhibit autoactivation?

is your bait a transcription factor?

[shashababy]

eldon, on Nov 25 2009, 03:50 PM, said:

shashababy, on Nov 25 2009, 04:42 AM, said:

Hey everyone:
      I have some troubles in my yeast two hybrid experiments.  Becasue my bait is self-activation , even after contransformed with prey, should all  the results   turn blue  in Theoretically? But when i cotransformed  my bait and preys,  the result is not all turn blue. （the same bait vs several preys）    Why?

the prey interacts to inhibit autoactivation?

is your bait a transcription factor?

my bait is a 35AA peptide. This peptide  can be autoactivation  , either it fusioned to AD or BD.   more interestiong,  it can be autoactivation, even without AD or BD. too strange. there is no such short transcription factor  reported before..........................

[willmen]

my bait also had self activation. by the domain analysis, i found that my protein has one SATA domain. In general, this domain has the function as one transcription factor. could i continue to do y2h via my protein