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yeast two hybrid problem


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#1 shashababy

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Posted 25 November 2009 - 04:42 AM

Hey everyone:
I have some troubles in my yeast two hybrid experiments. Becasue my bait is self-activation , even after contransformed with prey, should all the results turn blue in Theoretically? But when i cotransformed my bait and preys, the result is not all turn blue. (the same bait vs several preys) Why?

#2 eldon

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Posted 25 November 2009 - 03:50 PM

Hey everyone:
I have some troubles in my yeast two hybrid experiments. Becasue my bait is self-activation , even after contransformed with prey, should all the results turn blue in Theoretically? But when i cotransformed my bait and preys, the result is not all turn blue. (the same bait vs several preys) Why?

the prey interacts to inhibit autoactivation?

is your bait a transcription factor?

#3 shashababy

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Posted 25 November 2009 - 10:48 PM

Hey everyone:
I have some troubles in my yeast two hybrid experiments. Becasue my bait is self-activation , even after contransformed with prey, should all the results turn blue in Theoretically? But when i cotransformed my bait and preys, the result is not all turn blue. (the same bait vs several preys) Why?

the prey interacts to inhibit autoactivation?

is your bait a transcription factor?


my bait is a 35AA peptide. This peptide can be autoactivation , either it fusioned to AD or BD. more interestiong, it can be autoactivation, even without AD or BD. too strange. there is no such short transcription factor reported before..........................

#4 willmen

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Posted 28 December 2009 - 08:26 PM

my bait also had self activation. by the domain analysis, i found that my protein has one SATA domain. In general, this domain has the function as one transcription factor. could i continue to do y2h via my protein




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