3 replies to this topic

[bobsong]

Previous studys have shown that protein A and B located on ER membrane. Now I want to test whether, after treatment of a hormone, protein A will upregulate the activity of protein B by modifying it.

But I don't know how to do it. I'd appreciate your kind advice very much!

[Vini]

hey, what is the type of modification that you are looking for? Could you give in more details??

[bobsong]

DRN, on Nov 25 2009, 02:16 PM, said:

hey, what is the type of modification that you are looking for? Could you give in more details??

I'm  actually not sure about the existence of such modification. I predict there might be certain interaction between the two proteins on the ER membrane, based on some physiological phenomena. Phosphorylation perhaps, but i can't rule out other possibilities.

looking forward to your advice! thanks.

Edited by bobsong, 26 November 2009 - 01:11 AM.

[Vini]

bobsong, on Nov 26 2009, 02:38 PM, said:

DRN, on Nov 25 2009, 02:16 PM, said:

hey, what is the type of modification that you are looking for? Could you give in more details??

I'm  actually not sure about the existence of such modification. I predict there might be certain interaction between the two proteins on the ER membrane, based on some physiological phenomena. Phosphorylation perhaps, but i can't rule out other possibilities.

looking forward to your advice! thanks.

Hi, If I were in your place, I guess I would first look for protein homologies.......do a blast search....try to figure oyt putative domains........n lets say Phosphoryaltion is what u r looking for, then first put up an in vitro assay to establish this produc-substrate relationship. If this works out.........go for in vivo tests with radiolabeled phosphate to see increase in incorporation of phosphate in response to hormone...........of course, this is a rough sketch....hope this can act as a primer.......all the best

Edited by DRN, 26 November 2009 - 01:32 AM.