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how to explore protein interactions


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#1 bobsong

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Posted 24 November 2009 - 06:57 PM

Previous studys have shown that protein A and B located on ER membrane. Now I want to test whether, after treatment of a hormone, protein A will upregulate the activity of protein B by modifying it.

But I don't know how to do it. I'd appreciate your kind advice very much!

#2 Vini

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Posted 24 November 2009 - 10:16 PM

hey, what is the type of modification that you are looking for? Could you give in more details??

#3 bobsong

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Posted 26 November 2009 - 01:08 AM

hey, what is the type of modification that you are looking for? Could you give in more details??


I'm actually not sure about the existence of such modification. I predict there might be certain interaction between the two proteins on the ER membrane, based on some physiological phenomena. Phosphorylation perhaps, but i can't rule out other possibilities.

looking forward to your advice! thanks.

Edited by bobsong, 26 November 2009 - 01:11 AM.


#4 Vini

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Posted 26 November 2009 - 01:31 AM

hey, what is the type of modification that you are looking for? Could you give in more details??


I'm actually not sure about the existence of such modification. I predict there might be certain interaction between the two proteins on the ER membrane, based on some physiological phenomena. Phosphorylation perhaps, but i can't rule out other possibilities.

looking forward to your advice! thanks.



Hi, If I were in your place, I guess I would first look for protein homologies.......do a blast search....try to figure oyt putative domains........n lets say Phosphoryaltion is what u r looking for, then first put up an in vitro assay to establish this produc-substrate relationship. If this works out.........go for in vivo tests with radiolabeled phosphate to see increase in incorporation of phosphate in response to hormone...........of course, this is a rough sketch....hope this can act as a primer.......all the best

Edited by DRN, 26 November 2009 - 01:32 AM.





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