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[TINA JAMES]

I am Having a problem with severe filter background on a DIG labelled RNA probe.No matter what conc:of probe I use the background is really bad.Can anyone suggest a cause and solution to my problem.

[anonymous]

How are you labelling your RNA i think you mean cDNA from RNA right?  Back ground problems tend to arise when your membrane gets dry in the CDP-STAR™ stage.  Try increasing the amount of volume you use.  Also, try a more stringent SDS % solution which may help to reduce the peskie background.