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DIG RNA PROBES


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#1 TINA JAMES

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Posted 12 July 2001 - 09:00 PM

I am Having a problem with severe filter background on a DIG labelled RNA probe.No matter what conc:of probe I use the background is really bad.Can anyone suggest a cause and solution to my problem.

#2 anonymous

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Posted 13 July 2001 - 09:00 PM

How are you labelling your RNA i think you mean cDNA from RNA right? Back ground problems tend to arise when your membrane gets dry in the CDP-STAR™ stage. Try increasing the amount of volume you use. Also, try a more stringent SDS % solution which may help to reduce the peskie background.




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