I am Having a problem with severe filter background on a DIG labelled RNA probe.No matter what conc:of probe I use the background is really bad.Can anyone suggest a cause and solution to my problem.
DIG RNA PROBES
1 reply to this topic
Posted 13 July 2001 - 09:00 PM
How are you labelling your RNA i think you mean cDNA from RNA right? Back ground problems tend to arise when your membrane gets dry in the CDP-STAR stage. Try increasing the amount of volume you use. Also, try a more stringent SDS % solution which may help to reduce the peskie background.