2 replies to this topic

[Maddie]

The title says it all. Embarassing to admit I still don't know that, but, hey better to learn than stay an idiot, right?
So what is the difference between these 2? And what is the Purest?

My primers are between 30 and 37bp with a Biotin at the 5' end.

Here is what Invitrogen says:
HPLC: Guarantees highly purified primer required in some applications (=>85% full-length).
PAGE: Provides the highest percentage of full-length oligos (>=85%) required for certain demanding applications such as mutagenesis or adapter production
   huh?

Edited by Maddie, 24 November 2009 - 08:57 AM.

[Maddie]

Oh and maybe I should have said that it's urgent because I need to place a huge primer order (almost 200) asap.

[Maddie]

OKeeeeeeeeeeee, looks like I'm not the only ignorant around here  

So I give you my findings. HPLC and PAGE don't use the same tecnhique to separate according to lenght/molecular weight/charge and from what I gathered, HPLC's purity is between 90-97%, wherease PAGE is between 95-99%.
They cost the same (at least with Sigma), so I doubt the difference is big anyway.

Ahhh don't I love talking to myself