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Page or HPLC primers


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#1 Maddie

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Posted 24 November 2009 - 07:56 AM

The title says it all. Embarassing to admit I still don't know that, but, hey better to learn than stay an idiot, right?
So what is the difference between these 2? And what is the Purest? :angry:

My primers are between 30 and 37bp with a Biotin at the 5' end.

Here is what Invitrogen says:
HPLC: Guarantees highly purified primer required in some applications (=>85% full-length).
PAGE: Provides the highest percentage of full-length oligos (>=85%) required for certain demanding applications such as mutagenesis or adapter production
:angry: huh?

Edited by Maddie, 24 November 2009 - 08:57 AM.

Theory is when we know everything and nothing is working. Practice is when everything is working and nobody knows why. Here, we combine theory and practice. Nothing is working and nobody knows why.

A. Einstein

#2 Maddie

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Posted 24 November 2009 - 08:42 AM

Oh and maybe I should have said that it's urgent because I need to place a huge primer order (almost 200) asap.
Theory is when we know everything and nothing is working. Practice is when everything is working and nobody knows why. Here, we combine theory and practice. Nothing is working and nobody knows why.

A. Einstein

#3 Maddie

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Posted 25 November 2009 - 07:12 AM

OKeeeeeeeeeeee, looks like I'm not the only ignorant around here :blink:

So I give you my findings. HPLC and PAGE don't use the same tecnhique to separate according to lenght/molecular weight/charge and from what I gathered, HPLC's purity is between 90-97%, wherease PAGE is between 95-99%.
They cost the same (at least with Sigma), so I doubt the difference is big anyway.

Ahhh don't I love talking to myself :lol:
Theory is when we know everything and nothing is working. Practice is when everything is working and nobody knows why. Here, we combine theory and practice. Nothing is working and nobody knows why.

A. Einstein




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