Hi,
just to make sure: if I want to bisulphite sequence the reverse strand of my piece of DNA I can just design the primers according to the reverse complement of my forward strand, right?
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#1
ForEpi
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Posted 24 November 2009 - 07:36 AM
I believe in the power of Selenium!!
#2
methylnick
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Posted 24 November 2009 - 12:56 PM
yes the reverse complement of the forward strand PRIOR to mock bisulphite conversion.
ie: take your original genomic DNA sequence, reverse complement and THEN mock bisulphite treat using find and replace in word.
ie: take your original genomic DNA sequence, reverse complement and THEN mock bisulphite treat using find and replace in word.
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Posted 24 November 2009 - 11:08 PM
Cheers Nick.
I believe in the power of Selenium!!
