Suitable controls for for cellular fractionation
Posted 24 November 2009 - 06:57 AM
I am performing an cellular fractionation experiment where I have isolated both nuclear and cytosolic fractions from Jurkat cells. I am pretty sure that the technique is working well but I need appropriate antibody controls looking for proteins found exclusively in the nucleus/cytosol (particularly the cytosol). I have used an anti-lamin B antibody to confirm that the nuclear fraction contains this protein but would like another control to be sure (plus i have run out of the antibody and have no more funds ).
Thanks in advance,
Posted 24 November 2009 - 03:49 PM
Posted 05 December 2009 - 10:36 PM
purin consoude - comment parler en public