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Phenol chloroform ratio during RNA isolation


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#1 Vini

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Posted 24 November 2009 - 02:51 AM

what ratio of phenol: chloroform is used during RNA isolation? Searched in google and got different opinions...Few use 1:1 while others 5:1....which is appropriate and why? thanks for any suggestions :P

Edited by DRN, 24 November 2009 - 02:53 AM.


#2 molgen

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Posted 26 November 2009 - 03:14 AM

It all has to do with polarity and ionic strength.
Usually 1:1 ratio is used. When there is a need for one of the above reasons, you change the ratio.




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